Oral Histology & Development

Dentin Characteristics


Incremental lines
:

Von Ebner lines

Appearance:
  • Banding along the dentinal tubules 
  • Perpendicular to dentinal tubules

Cause:
  • The dentine formation occurs incrementally ~ 4mm / day.
  • A change of collagen fiber orientation was seen more clearly after 5-day cycle of this daily deposition  
Contour lines of Owen
Appearance:
In longitudinal ground sections, they appeared as dark bands. 

Cause:
  • Changes in the coloration of the dentin
  • Periods of illness
  • Changes of body metabolism or inadequate nutrition

Tom's granular layer:

Appearance:
  • Black granules.
  • Only clear in ground section. 
  • Increasing in the granules occurs from the CEJ to the apex of the tooth
  • Visible in radicular (root) dentin near DCJ

Cause:
  • Looping back of dentinal tubules
  • Special arrangement of collagen & non- collagenous proteins between dentin and cementum


Interglobular dentine:

Appearance:
  • This pattern of mineralization is best seen in circumpulpal dentin just below mantel dentin, because of the globular pattern of mineralization in this region
  • In a ground section of dentin, the less-calcified areas of dentin appear as irregularly shaped crescents

Cause:

  • Areas of unmineralised or hypomineralised dentin, where globular zones of mineralization defect to fuse into a homogenous mass within mature dentin
  • Common in persons with Vitamin D deficiency or high levels of Fl- at the time of dentinogenesis


Changes in dentinal tubules:

Dead tracts

Appearance: 
  • Appears dark in ground sections
  • Mostly in the coronal Dentine

Cause:

  • They occur as age change
  • A severe injury or stimulus causes the odontoblasts to be destroyed in the region
  • When dentin is damaged, odontoblastic processes die or retract leaving empty dentinal tubules

Sclerotic dentine
Appearance:
  • Dentin appeared glassy and becomes translucent
  • Is most common in the apical third of the root

Causes:

  • Increase with age
  • Due to noxious stimuli as in chronic caries
  • Irritated odontoblasts forms sclerotic dentin as a protective wall